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proline-rich motif of N-terminal portion of the S-A1 S antigen (S-A1SAg) is essential for oncogenic transformation.
Previous studies showed that the S-A1SAg S antigen (S-A1S Ag) is a potent oncogene (S-A1S Ag-transformed NIH 3T3 cells form tumors in nude mice). In this study, we characterized the S-A1S Ag protein by expressing each S-A1S Ag isoform from a bicistronic mRNA containing the bicistronic signal of the cytomegalovirus. The expressed proteins were detected by anti-S-A1S Ag antibody recognizing not only the endogenous S-A1S Ag but also the S-A1S Ag expressing cells. We found that the N-terminal portion of S-A1S Ag (S-A1S Ag-N) is involved in oncogenic transformation. Antibodies against S-A1S Ag-N or S-A1S Ag-C inhibited the S-A1S Ag-transformed NIH 3T3 cell growth, and S-A1S Ag-N inhibited the S-A1S Ag-transformed cell growth in nude mice. Analyses of the DNA binding activities of the recombinant S-A1S Ag-N and its C-terminal portion (S-A1S Ag-C) by electrophoretic mobility shift assay suggested that S-A1S Ag-N is involved in the activation of its target genes. These results suggest that the N-terminal proline-rich motif of S-A1S Ag is involved in the oncogenic transformation and that the DNA binding activity of S-A1S Ag-N could be essential for oncogenic transformation.Q:
What features of a MOSFET are important when selecting a gate driver?
Is the number of fingers that the gate must be driven by important, the amount of input current (output current), gate capacitance, the duration a gate is held high, or something else?
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The gate capacitance is more important.
The smaller the gate capacitance the lower the gate drive voltage for a given input voltage.
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